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KMID : 0378019760190040511
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New Medical Journal
1976 Volume.19 No. 4 p.511 ~ p.519
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Histochemical Studies on the AS-Positive Cells of the Thymus in Mammals
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Abstract
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As a preliminary experiment for the studies of the cells forming the microenvironment of the thymus the present experiment have been examined for the appearance, the localization and the histochemical properties of the PAS-positive cells in the thymuses of rats, mice and rabbits.
After animals of various ages (from 1st week to 40th week after birth) were sacrificed the thymus were removed from each animals. The serial paraffine sections were carried out for the staining with PAS, M. G. P., toluidine blue and HE.
At one month after birth, in all of the experimental animals a few of the PAS-positive cells appeared at the corticomedullary junction of the thymuses and the number of these cells increased with advancing age at the same area. In the animals after 6th month, most of the PAS-positive cells localized at the subcapsular area in the mice and at the corticomedullary junction in the rats and rabbits.
The size of the PAS-positive cells increased progressively with advancing age and the cytoplasm of these cells packed with the granules which gave positive reactions to PAS and pyronine and negative reaction to toluidine blue.
Frequently the mitotic lymphocytes which were uniformly scattered throughout the cortex were seen in each thymic lobules of all experimental animals, while the PAS-positive cells were not observed in some of thymic lobules in the animals before 4 month old.
Those results suggested that the PAS-positive cells may be related with differentiation or maturation rather than proliferation of the thymic lymphocytes.
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KEYWORD
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